Composite

Part:BBa_K2235006

Designed by: Shivashree Dhanaraj, Shanlin Tong, Gilai Nachmann and Sina Amoor pour   Group: iGEM17_Stockholm   (2017-10-11)


Sialidase enzyme with a RBS attached

This part constitutes of RBS functional unit attached to sialidase basic part (BBa_K2235005) that can be used to test on various promoters. BBa_K2235007 biobrick has the characterisation for an OmpR promoter fused to this (BBa_K2235006) part.
The following biobrick parts contain the sialidase enzyme:
Basic part BBa_K2235005 consists of the sialidase enzyme coding sequence with a His tag C-terminally attached.
BBa_K2235009 biobrick is a composite of a T7 promoter and RBS followed by sialidase with characterisation data of the enzyme.
BBa_K2235011 is a conjugation of sialidase composite (BBa_K2235009) with a device that facilitates the secretion of the enzyme out of the E.coli bacterial cell.

Sequence and Features

Sialidase enzyme sequence has a 6XHis-tag N-terminally attached.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 73
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
    Illegal NgoMIV site found at 595
    Illegal NgoMIV site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1065


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Categories
Parameters
None